Fig. 7.

CL-43 reduced the cytoprotective effect caused by monocyte-like cells inhibiting HSF1 activity. A HCC cells were incubated with 500 nm CL-43, co-cultivated with THP1 and, 20 h later, subjected to western blotting with antibodies against HSF1^Ser326 and Hsp70. Band intensity was measured with the aid of the ImageJ program. Numbers indicate a ratio between HSF1 or Hsp70 band intensity to band intensity of tubulin. B HCC cells were seeded on E-plates and treated with 500 nM CL-43 in combination with 75 μM CQ in the presence or absence of THP1. The signal was recorded using the xCELLigence system for 30–40 h