Table 2.
Number of regions completely covered by the ME49 replicates used to assess the analytical sensitivity of the Ion AmpliSeq method after mapping to a ME49 reference genome (ToxoDB release 53). Coverage is shown in relation to the different dilutions and number of cycles used in the Ion AmpliSeq multiplex PCR
| Dilution | PCR cycles | Completely covered regions, relative to a ME49 reference genome | ||
|---|---|---|---|---|
| ME49 Ion AmpliSeq replicate 1 | ME49 Ion AmpliSeq replicate 2 | ME49 Ion AmpliSeq replicate 3 | ||
| 10(-1) | 24 | 17 | 17 | 17 |
| 10(-1) | 26 | 17 | 17 | 17 |
| 10(-1) | 28 | 17 | 17 | 17 |
| 10(-2) | 24 | 17 | 17 | 17 |
| 10(-2) | 26 | 17 | 17 | 17 |
| 10(-2) | 28 | 17 | 17 | 17 |
| 10(-3) | 24 | 12 | 10 | 9 |
| 10(-3) | 26 | 14 | 13 | 9 |
| 10(-3) | 28 | 16 | 15 | 14 |