Figure 10.

Biological characterization of a bioprinted GCG in vivo. (a) Printed acellular structure (spiral) in the GCG supporting hydrogel. (b) 3D construct in the GCG supporting bath subcutaneously implanted in the back pocket of a rat for 1 week. (c, d) H&E-stained horizontal histological slices of the explanted printed construct. The representative histology slices presented are from different places along the thickness of the explant, showing the printed structure occupying different positions in space owing to its spiral architecture. (e) 3D volume mesh of the explant slices rendered by the SIRT volumetric algorithm revealing a spiral structure of the explanted printed construct. White dotted lines show the respective volume space in the mesh that is contributed by the histology slices on the left.