Figure 7.

Cellular prints of muscle fiber mimic 3D tissues. (a–c) Schematics and printed construct of a muscle fiber mimic tissue with a core–shell geometry containing two cell types. The core contains C2C12 myocytes, and shell contains HUVECs. (d) Photograph and fluorescent microphotographs of the muscle fiber mimic after 21 days of culture. e, Immunofluorescent images show the differentiation of C2C12 cells into proliferating muscle cells, as depicted by f-actin and myosin heavy chain (MHC) staining. HUVECs exhibited a tendency to make microvasculature within different individual fibers in the muscle fiber mimic tissue, as depicted by CD31 staining.