TABLE 2.
E. coli fluorescence in the presence of HLP 2 and CCCP or in the presence of HLP 2 at 4°C determined by using DiBAC4(3) and PI
| Conditiona | Fluorescenceb
|
|||
|---|---|---|---|---|
| Without HLP 2
|
With HLP 2
|
|||
| DiBAC4(3) | PI | DIBAC4(3) | PI | |
| CCCP | ||||
| Absent | 6.7 | 3.3 | 98.3 | 99.4 |
| Present | 46.0 | 54.2 | 90.2 | 95.9 |
| Temp (°C) | ||||
| 37 | 6.4 | 1.2 | 96.3 | 98.2 |
| 4 | 24.7 | 15.5 | 94.1 | 97.2 |
a
E. coli grown to log phase was used to give a starting inoculum of 108 bacteria/ml and incubated for 2 h in the presence or absence of HLP 2 and either with or without CCCP or at 4 or 37°C.
b
Fluorescence was monitored by flow cytometry and is expressed as the percentage of fluorescent cells in the whole population. Results are means from at least four experiments.