Table 2.

Spiking recovery. Two samples with different amounts of analytes were mixed in different ratios and recovery, expressed as a percentage, was calculated for each dilution for comparison of expected versus measured concentrations

	Volume, %
	Sample 1	Sample 2	Expected	Observed	%
Adiponectin	100	0	5553.40	5553.40	100
	75	25	4612.25	5134.75	111.3
	62.5	37.5	4141.67	4591.60	110.9
	50	50	3671.09	4095.93	111.6
	37.5	62.5	3200.52	3525.41	110.2
	25	75	2729.94	2951.53	108.1
	0	100	1788.79	1788.79	100
Ghrelin	100	0	109.07	109.07	100
	75	25	92.85	104.14	112.2
	62.5	37.5	84.74	76.92	90.8
	50	50	76.63	79.84	104.2
	37.5	62.5	68.52	73.16	106.8
	25	75	60.41	59.90	99.1
	0	100	44.19	44.19	100
IGF-1	100	0	1562.00	1562.00	100
	75	25	1222.75	1288.00	105.3
	62.5	37.5	1053.13	1155.00	109.7
	50	50	883.50	992.00	112.3
	37.5	62.5	713.88	747.00	104.6
	25	75	544.25	551.00	101.2
	0	100	205.00	205.00	100