FIG. 4.
C3b affinity chromatography of membrane protein extracts derived from transfected T. cruzi epimastigotes. Epimastigotes were metabolically labeled with [35S]methionine, and detergent-solubilized membrane protein extracts were subjected to C3b affinity chromatography. Eluted proteins were fractionated by SDS-PAGE, and the gel was prepared for fluorography and exposed to X-ray film for 24 h. Lanes: 1, C3b-eluted protein from tissue culture-derived T. cruzi trypomastigotes; 2, transfected CRP 2.3 epimastigotes; 3, pTEX-transfected epimastigotes. Molecular mass standards (kilodaltons) are shown at the left.