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. 2024 Jan 10;6(1):141–152. doi: 10.1038/s42255-023-00948-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a Schematic overview of experimental setup. Osteoclasts were treated with the JMJD3 inhibitor GSK-J4 during the entire experiment. b, c TRAP staining (b) and quantification of TRAP⁺ MNCs (c) from wild-type mice treated with either vehicle or GSK-J4 (n = 4; unpaired, two-tailed Student’s t-test). d Phgdh, Csf1r, Tnfrsf11a (encoding for RANK), Nfatc1 (P = 0.007), cMyc, Calcr (P = 0.015), Acp5 (P = 0.007), Ctsk (P = 0.008), Dcstamp (P = 0.02), Atp6v0d2 (P = 0.04), Mmp9 (P < 0.0001) and Irf8 mRNA levels in osteoclasts treated with either vehicle or GSK-J4 (n = 4; unpaired, two-tailed Student’s t-test with *p < 0.05 vs vehicle). e Schematic overview of experimental setup. Osteoclasts were treated with ascorbate during early osteoclast differentiation (that is from d0 to d3). f-g TRAP staining (f) and quantification of TRAP⁺ MNCs (g) from Phgdh^oc+ and Phgdh^oc- mice treated with vehicle or ascorbate (n = 4; two-way ANOVA). Scale bar is 50 µm. Data are means ± s.d.

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