Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jan 10;6(1):141–152. doi: 10.1038/s42255-023-00948-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 1 — a Representative pictures of osteoclast differentiation at indicated timepoints. Black and yellow arrowheads indicate osteoclasts. Scale bar is 50 µm. Representative pictures of 4 biological replicates are shown. b Schematic of carbon atom (circles) transitions of [¹³C₆]glucose used to detect label incorporation into indicated metabolites. G6P is glucose-6-phosphate, F6P is fructose-6-phosphate, 3PG is 3-phosphoglycerate, Ac-CoA is acetyl-CoA, aKG is α-ketoglutarate. c-e G6P/F6P (c), pyruvate (Pyr; d) lactate (Lac; e) labeling from [¹³C₆]glucose at different timepoints during osteoclast differentiation (n = 4; one-way ANOVA). f, g Relative lactate production (f) and ratio of lactate production over glucose consumption (g) (n = 4; one-way ANOVA). h-m Citrate (Cit; h), aKG (i), succinate (Suc; j), fumarate (Fum; k), malate (Mal; l) and ribose-5-phosphate (R5P; m) labeling from [¹³C₆]glucose (n = 4; one-way ANOVA). Data are means ± s.d. Relevant mass distribution vectors (MDVs) are shown in (c-e) and (h-m).

Source data