FIG. 4.

Effects of sample volume and an albumin carrier. Seven replicate radial diffusion experiments were done, using either 5- or 8-μl samples that had been serially diluted in the presence or absence of 0.1% human serum albumin (HSA). Note the effects of these changes on the MIC, which corresponds to the x intercept. The regression lines for the data points (least-mean-squares fit) are as follows: solid line, 5 μl without HSA; dashed line, 8 μl without HSA; dotted line, 8 μl with HSA.