TABLE 5.
Activities of truncated protegrins against C. albicans UC820a
| Prote- grin | Deletions (positions) | MICa (μM) | MICb (μM) | Lethal concn (μM) | Ratio |
|---|---|---|---|---|---|
| PG-1 | None | 1.68 ± 0.10 | 1.53 ± 0.08 | 0.28 ± 0.02 | 1.00 |
| PC-13 | 17, 18 | 2.23 ± 0.13* | 2.77 ± 0.18** | 0.70 ± 0.03** | 0.75 |
| PC-45 | 16–18 | 5.60 ± 0.43** | 5.54 ± 0.40** | 1.15 ± 0.07** | 0.30 |
| PC-11 | 1–4 | 6.27 ± 0.30** | 6.92 ± 0.30** | 1.24 ± 0.05** | 0.27 |
| PC-37 | 1–5 | 17.1 ± 0.44** | 16.0 ± 0.55** | 5.46 ± 0.19** | 0.10 |
| PC-17 | 1–4, 17, 18 | 7.04 ± 0.46** | 7.58 ± 0.39** | 1.36 ± 0.04** | 0.24 |
| PC-73 | 1–5, 16–18 | >103.7** | >103.7** | >103.7** | <0.02 |
a
Radial diffusion assays were performed as described in Table 2, footnote a. Values represent means ± SEMs (n = 3 experiments, except for PG-1 [n = 7]). Deleted residue positions are keyed to PG-1. See Table 1 for more details. The ratio was calculated by dividing the MICa of PG-1 by the MICa of the peptide of interest, shown in column 1. Values were compared with those for PG-1 by unpaired t tests: ∗, P < 0.05; ∗∗, P < 0.01. The mean values for PC-73 were well outside the 99% confidence limits for PG-1. The r values for the regression lines used to calculate the MICa values ranged from 0.979 ± 0.012 to 0.999 ± 0.000.