FIG. 5.
Myeloperoxidase assay of PMNs in the monolayers (black bars) and reservoirs (hatched bars) after 2 h of transmigration. Grey bars, total. T84 monolayers were incubated with no CNF1 (bars 0) or with 10−10 M (bars 1), 10−11 M (bars 2), or 10−12 M (bars 3) CNF1. (A) Transmigration of PMNs in the physiological (basolateral-to-apical) direction was diminished in a concentration-dependent manner in the presence of CNF1. (B) Transmigration of PMNs in the nonphysiological (apical-to-basolateral) direction was also attenuated in a concentration-dependent manner in monolayers incubated with CNF1. Data are pooled from 6 to 12 individual monolayers for each condition, and results are expressed as means ± SEM.