MAP-2 immunostaining of the hippocampus in a rat injected with the vehicle alone (A through C) or 50 ng of epsilon-toxin per kg (D through F). The rats were sacrificed at 4 h p.i. Panels B and C are higher magnifications of the CA1 and CA3 subfields, respectively, in panel A. Panels E and F are higher magnifications of the CA1 and CA3 subfields, respectively, in panel D. Note that MAP-2 immunoreactivity was lost in the dendrite and perikarya of dark cells (D, E, and F). Bars, 500 μm (A and D) and 50 μm (B, C, E, and F).