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. 2024 Jan 9;9(1):99–107. doi: 10.1016/j.synbio.2024.01.003

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© 2024 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — Experimental flowchart of this study. GAMT: Guanidinoacetate N-methyltransferase; gamt: Guanidinoacetate N-methyltransferase gene; McGAMT: Guanidinoacetate N-methyltransferase from Mus caroli (Ryukyu mouse); AcGAMT: Guanidinoacetate N-methyltransferase from Antrostomus carolinensis (chuck-will's-widow); CMR: Chloramphenicol-resistance gene; LacIq: Lac repressor encoding gene; ORI: Origin of replication; PBLi: P1-like Bacteriophage; MCS: Multiple cloning site; GAA: Guanidinoacetic acid; SAM: S-adenosyl methionine; SAH: S-adenosyl-l-homocysteine; MET: Methionine; ATP: Adenosine triphosphate; Tac: Tryptophan operon repressor and activator protein-inducible and catabolite-repressible promoter.