TABLE 2.
Sensitivity of PCR and RIT in detection of T. pallidum in tissues from nonimmune infected (NI/TP) and immune reinfected (I/TP) rabbits
| Rabbit group | Rabbit no. | Days after inf. or reinf.a | Test resultsb for tissues from:
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Skin
|
Testes
|
ILN | SP | HRT | BRN | |||||
| PCR | RIT | PCR | RIT | |||||||
| NI/TP | 4 | 15 | ND | ND | ND | ND | + | + | + | + |
| 6 | 60 | 6/10 | + | 7/16 | + | + | + | ND | ND | |
| 7 | 100 | 6/10 | + | 6/16 | + | + | + | + | + | |
| 8 | 120 | 1/10 | − | 6/16 | + | + | + | + | + | |
| I/TP | 14 | 1 | 10/10 | + | 9/15 | + | − | − | − | − |
| 15 | 7 | 8/10 | + | 3/15 | + | − | − | − | − | |
| 16 | 15 | 9/10 | + | 0/16 | − | − | − | − | − | |
| 17 | 21 | 3/10 | − | 0/16 | − | − | − | − | − | |
| 18 | 30 | 2/10 | − | 0/16 | − | − | − | − | − | |
| 19 | 50 | 0/10 | − | 0/16 | − | − | − | − | − | |
a
inf., infection; reinf., reinfection.
b
+, positive; −, negative; ND, not determined. Values represent number of positive samples detected relative to the total number examined. ILN, SP, HRT, and BRN samples were tested by PCR alone.