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. 2024 Jan 18;121(4):e2305745121. doi: 10.1073/pnas.2305745121

Fig. 1.

Fig. 1.

Genetic construction and detection of Osr2HN. (A) Schematic diagram of antigen design for Osr2HN vaccine. A head-to-tail antigen model was proposed to form HN tetramers and mimic the natural structure of the major protective antigen (HN protein) in paramyxovirus. This design facilitates the formation of HN tetramers and epitope exposure, providing enhanced protection. (B) Schematic of pCAMBIA 1300-2HN plasmid. RB, right border; Gt13a promoter, rice seed storage protein glutelin gene promoter; SP, Gt13a signal peptide; Nos, nopaline synthase gene terminator; LB, left border; Osr2HN, two HN linked by (GGGGS)3. (C) SDS-PAGE analysis of Osr2HN and OsrHN expressed in rice seeds. (D) Expression level of Osr2HN in different transgenic lines (P2-3, P2-90, P2-101, P2-97, P2-1, and P2-2) and the nontransgenic control (TP309). Mean values from three independent experiments are shown, with error bars representing SD. (E) Subcellular localization of Osr2HN in rice endosperm cells was detected using anti-HN antibody staining. (F) Stable expression of Osr2HN in transgenic line P2-3. G1, G2, G3, and G4 are the first, second, third, and fourth generations of P2-3, respectively.