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. 2023 Nov 16;115(1):155–169. doi: 10.1111/cas.16014

FIGURE 4.

FIGURE 4

MicroRNA (miR)‐296‐3p directly targets phosphatase and tensin homolog (PTEN) and suppressor of cytokine signaling 6 (SOCS6). (A) Schematic of the constructed luciferase reporter plasmid constructed containing WT and mutant (Mut) binding sites in the 3′‐UTR of PTEN and SOCS6 mRNA. (B) Analysis of dual‐luciferase reporter assays revealed the interaction between miR‐296‐3p with PTEN and SOCS6. (C) Relative mRNAs expressions of PTEN and SOCS6 were analyzed by real‐time PCR in A2780 and SKOV3 cells. (D) Western blot analysis of PTEN and SOCS6 protein levels in A2780 and SKOV3 cells. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. ns, no significance.