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. 2023 Nov 16;115(1):155–169. doi: 10.1111/cas.16014

FIGURE 5.

FIGURE 5

MicroRNA (miR)‐296‐3p mediates the proliferation, migration, and invasion of ovarian cancer cells in part through downregulation of phosphatase and tensin homolog (PTEN) and suppressor of cytokine signaling 6 (SOCS6). (A) Western blot analysis of PTEN and SOCS6 expression in A2780 and SKOV3 cells with PTEN or SOCS6 overexpression (OE). (B–E) Ectopic PTEN or SOCS6 expression in A2780 and SKOV3 cells. (B) Cell proliferation, (C) colony formation, (D) migration, and (E) invasion abilities were investigated by EdU, colony formation, Transwell migration, and invasion assays. (F) Western blot analysis of PTEN and SOCS6 levels in A2780 and SKOV3 cells cotransfected with miR‐296‐3p and PTEN or SOCS6. (G) Cell proliferation and (H) colony formation of A2780 and SKOV3 cells with PTEN or SOCS6 overexpression combined with or without miR‐296‐3p mimic. (I,J) Cell migration and invasion of A2780 and SKOV3 cells determined by Transwell assays. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. miR‐NC, mimic negative control.