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. 2023 Nov 16;115(1):155–169. doi: 10.1111/cas.16014

FIGURE 6.

FIGURE 6

MicroRNA (miR)‐296‐3p activates downstream AKT and signal transducer and activator of transcription 3 (STAT3) signaling pathways by targeting phosphatase and tensin homolog (PTEN) and suppressor of cytokine signaling 6 (SOCS6), respectively. (A, B) Western blot analysis of the critical mediators of AKT and STAT3 signaling pathways, including AKT, p‐AKT (S473), p‐AKT (T308), STAT3, and p‐STAT3 in A2780 and SKOV3 cells transfected miR‐296‐3p or anti‐miR‐296‐3p. (C) Overexpression (OE) of PTEN significantly reduced the p‐AKT (S473) and p‐AKT (T308) protein levels in A2780 and SKOV3 cells. (D) Relative protein expressions of AKT, p‐AKT (S473), and p‐AKT (T308) were analyzed by western blot in A2780 and SKOV3 cells cotransfected with PTEN and miR‐NC or miR‐296‐3p. (E) Western blot results demonstrate STAT3 and p‐STAT3 expression in A2780 and SKOV3 cells with SOCS6 overexpression. (F) Relative protein levels of STAT3 and p‐STAT3 were analyzed by western blot in A2780 and SKOV3 cells cotransfected with SOCS6 and miR‐NC or miR‐296‐3p. Band intensity was assessed with ImageJ. *p < 0.05; **p < 0.01; ***p < 0.001. ns, no significance. miR‐NC, mimic negative control.