Figure 8.

BAY1830389 (A) and BAY1834845 (B) dose-dependently blocked IL-1β-induced inflammation in mice. Female BALB/c mice were treated with vehicle, BAY1830839, or BAY1834845, followed by intraperitoneal injection of 90 μg/kg of IL-1β 6 h after treatment. After an additional 2 h, the animals were euthanized, and blood was collected to obtain plasma samples. IL-6 and TNF-alpha plasma levels were then determined by multiplex protein assay. Data are shown as mean ± SD for each treatment group (n = 5 per treatment group, except for vehicle alone [n = 4]; results for IL-6 levels in the 80 mg/kg BAY1834845 group [n = 3]). One-way ANOVA vs IL-1β vehicle: *p < 0.05, **p < 0.01, ***p < 0.005, and ****p < 0.001.