Fig. 8.

Expressed and upregulated genes detected in a pediatric diffuse midline glioma. A Correlation plot illustrating the fold-change in PacBio long-read count (x-axis) and fold-change in Illumina short-read transcript per million (TPM) value (y-axis) per isoforms identified in the tumor relative to the adjacent non-diseased tissue comparator sample. The genes represented in the correlation plot are the 1,799 transcripts that were found in the tumor sample with at least ten Circular Consensus Sequencing “CCS” long-reads of support and with short-read TPM value of ten or greater. B Heatmap of identified genes (y-axis) enriched in the tumor sample associated with their respective Reactome pathways (x-axis) wherein green indicates that gene is noted as being part of that pathway