Table 5.
Purification profile of Bitis arietans metalloproteinase, serine protease and phospholipase A2 fractions
| Venom fraction | Purification step | Total protein (µg) | Total activity (µmol/min) | Specific activity (µmol/min/mg) | Purification fold | Yield (%) |
|---|---|---|---|---|---|---|
| SVMP | ||||||
| Crude venom | 2.64 | 5.40 | 2.08 | 1.00 | 100.00 | |
| Acetone precipitation | 0.83 | 2.41 | 2.90 | 1.39 | 48.20 | |
| Ion exchange chromatography on DEAE cellulose | 0.40 | 1.62 | 4.05 | 1.95 | 30.00 | |
| Gel filtration on Sephadex G-75 | 0.16 | 0.85 | 5.31 | 2.55 | 15.75 | |
| SVSP | ||||||
| Crude venom | 2.64 | 4.73 | 1.79 | 1.00 | 100.00 | |
| Acetone precipitation | 0.93 | 2.54 | 2.73 | 1.52 | 53.69 | |
| Ion exchange chromatography on DEAE cellulose | 0.52 | 1.50 | 2.88 | 1.58 | 31.70 | |
| Gel filtration on Sephadex G-75 | 0.32 | 0.96 | 3.00 | 1.68 | 20.30 | |
| PLA2 | ||||||
| Crude venom | 2.64 | 2.98 | 1.13 | 1.00 | 100.00 | |
| Acetone precipitation | 1.62 | 2.24 | 1.38 | 1.22 | 77.24 | |
| Ion exchange chromatography on DEAE cellulose | 1.09 | 1.80 | 1.65 | 1.46 | 60.40 | |
| Gel filtration on Sephadex G-75 | 0.14 | 0.53 | 3.79 | 3.35 | 17.78 | |