. 2024 Jan 29;23:37. doi: 10.1186/s12934-023-02277-x

Table 3.

The guaranteed rate of succinate growth-coupled production in in iJR904 (Strain0 in CM2 cultivation medium)

Number of knocked out reactions	Deleted reactions	Biomass formation rate (h⁻¹)	Succinate production rate (mmol.gDW⁻¹ h⁻¹)		SoGC (h⁻¹)	Deleted genes	Co-knockout reactions
Number of knocked out reactions	Deleted reactions	Biomass formation rate (h⁻¹)	min	max	SoGC (h⁻¹)	Deleted genes	Co-knockout reactions
Single	ADHEr	0.16	5.11	9.50	1.41E-2	b1241	LCADi_copy2
Double	ADHEr, LDH_D	0.15	8.08	9.51	1.43E-2	b1241, b2133, b1380	LCADi_copy2
Triple	ADHEr, LDH_D, PFL	0.12	11.08	12.73	1.53E-2	b1241, b2133, b1380, b3114, b0902, b3951	LCADi_copy, OBTFL
Quadruple	ADHEr, LDH_D, PFL, THD2	0.11	12.29	13.01	2.58E-2	b1241, b2133, b1380, b3114, b0902, b3951, b1602	LCADi_copy, OBTFL
Quintuple	ADHEr, LDH_D, GLUDy, PFL, THD2	0.10	12.34	13.06	2.61E-2	b1241, b2133, b1380, b1761, b3114, b0902, b3951, b1602	LCADi_copy, OBTFL