Table 4.

The maximum rates of succinate growth-coupled production in iML1515 (Strain0 in CM2 cultivation medium)

Number of knocked out reactions	Deleted reactions	Biomass formation rate (h−1)	Succinate production rate (mmol.gDW−1 h−1)	Deleted genes	Co-Knockout reactions
Single	ATPS4rpp	0.25	12.73	b3735, b3737, b3738, b3732, b3733, b3736, b3734, b3731, b3739	–
Double	ATPS4rpp, PGL	0.24	16.54	b3735, b3737, b3738, b3732, b3733, b3736, b3734, b3731, b3739, b0767	–
Triple	PGI, ATPS4rpp, G6PDH2r	0.17	23.16	b4025, b3734, b3733, b3736, b3732, b3737, b3731, b3738, b3739, b3735, b1852	–
Quadruple	PFL, ACALD, THD2pp, THD2pp	0.19	23.49	b0351, b1241, b0903, b3951, b2579, b3952, b3114, b0902, b1602, b2913	OBTFL, 'ALCD2x', 'ALCD19'