Table 5.

The maximum rates of succinate growth-coupled production in iJR904 in rich medium (Strain0 in LB cultivation medium)

Number of knocked out reactions	Deleted reactions	Biomass formation rate (h−1)	Succinate production rate (mmol.gDW−1 h−1)	Deleted genes	Co-Knockout reactions
Single	ADHEr	1.35	20.10	b1241	LCADi_copy2
Double	F6PA, PFK	1.28	33.69	b0825, b3946, b3916, b1723	−
Triple	ACKr, GLCpts, PYK	0.56	54.88	b2296, b3115, b1849, b1819, b2415, b2416, b1621, b1101, b2417, b1817, b1818, b1854, b1676	DHAPT, GART, PPAKr
Quadruple	ACKr, ARGDC, GLCpts, PYK	0.56	64.72	b2296, b3115, b1849, b2938, b4117, b1819, b2415, b2416, b1621, b1101, b2417, b1817, b1818, b1854, b1676	GART, PPAKr, DHAPTs