Table 6.

The maximum rates of succinate growth-coupled production in iML1515 in rich medium (Strain0 in LB medium)

Number of knocked out reactions	Deleted reactions	Biomass formation rate (h−1)	Succinate production rate (mmol.gDW−1 h−1)	Deleted genes	Co-Knockout reactions
Single	ARGDC	1.08	19.72	b4117	–
Double	ARGDC, FADRx	1.05	22.09	b4117, b3844	FADRx, FE3Ri, FLVRx
Triple	NDPK5, ASPTA, ARGDC	1.03	28.14	b0474, b2518, b0928, b4054, b4117	ADK1, NDPK2, ADNK1, NDPK3, NDPK6, DADK, ADK4, NDPK1, ADK3, NDPK4, NDPK7, NDPK8, TYRTA, PHETA1, LEUTAi
Quadruple	NDPK5, PFL, LDH_D, ACALD	0.75	40.97	b0474, b2518, b2579, b3952, b0902, b3951, b0903, b3114, b1380, b0351, b1241	ADK1, NDPK2, ADNK1, NDPK3, NDPK6, DADK, ADK4, NDPK1, ADK3, NDPK4, NDPK7, NDPK8, OBTFL, ALCD2x, ALCD19