Table 7.
The best solutions based on the desired evaluation indexes for succinate production under anaerobic condition (Strain0 in CM2 cultivation medium) in iJR904
| Number of Knocked out reaction | Evaluation index | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SSP (h−1) (FBA-based calculations) |
SSP (h−1) (LinearMoMA-based calculations) [62]) |
SoGC (h−1) (FBA-based calculations) |
||||||||||
| Best knockout strategy | Biomass formation rate (h−1) | Succinate production rate (mmol g DW−1) | SSP (h−1) × 10 | Best knockout strategy | Biomass formation rate (h−1) | Succinate production rate (mmol g DW−1) | SSP (h−1) × 10 | Best knockout strategy | Biomass formation rate (h−1) | Succinate production rate (mmol g DW−1) | SSP (h−1) × 10 | |
| 1 | ADHEr | 0.16 | 0.83 | 0.14 | ADHEr | 0.03 | 2.38 | 0.08 | ADHEr | 0.03 | 2.38 | 1.34 |
| 2 | ADHEr, LDH_D | 0.16 | 8.73 | 1.43 | ADHEr, ATPS4r | 0.12 | 8.32 | 1.01 | ADHEr, LDH_D | 0.16 | 8.73 | 1.35 |
| 3 | ADHEr, LDH_D, PFL | 0.12 | 12.24 | 1.53 | ADHEr, ATPS4r, RPE | 0.13 | 8.60 | 1.19 | ADHEr, ATPS4r, LDH_D | 0.16 | 8.90 | 3.01 |
| 4 | ADHEr, LDH_D, PFL, URIK2 | 0.12 | 12.24 | 1.53 | ADHEr, ATPS4r, LDH_D, RPE | 0.13 | 8.71 | 1.20 | ADHEr, LDH_D, HEX1, THD2 | 0.13 | 9.88 | 3.09 |
| 5 | ADHEr, P, PFL, SUCOAS, RNDR3 | 0.12 | 12.25 | 1.54 | ADHEr, ATPS4r, GLYK, F6PA, RPE | 0.14 | 8.63 | 1.23 | ADHEr, LDH_D, HEX1, THD2, DRPA | 0.13 | 9.87 | 3.10 |