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. 2024 Jan 29;19:13. doi: 10.1186/s13024-023-00690-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — SARM1 knockdown restores APP axonal transport in NMNAT2 KO neurons (A1, B1, C1) Experimental timeline. (A2, B2, C2) Percentages of APP vesicles moving anterogradely, retrogradely, or staying stationary in distal axons of ctrl and KO neurons following the treatments indicated in A1, B1, and C1, respectively. Numbers of neurons imaged and statistics: (A2) 27 ctrl + ctrl ASO, 31 KO + ctrl ASO, 29 ctrl + SARM1 ASO, and 33 KO + SARM1 ASO, *p = 0.0286, ^#p = 0.018, **p = 0.0069. (B2) 20 ctrl + ctrl ASO, 22 KO + ctrl ASO, 14 ctrl + SARM1 ASO, and 26 KO + SARM1 ASO. (C2) 25 ctrl + ctrl ASO, 31 KO + ctrl ASO, 22 ctrl + SARM1 ASO, and 26 KO + SARM1 ASO, ^###p = 0.0006. Significant comparison to KO + ctrl ASO group was labeled with ^#. (A3-4, B3-4, C3-4) Velocity of antero- and retrograde transport in distal axons of ctrl and KO neurons following the treatments indicated in (A1, B1, C1), respectively. (A3-4) 26-27 ctrl + ctrl ASO, 26-27 KO + ctrl ASO, 29 ctrl + SARM1 ASO, and 32-33 KO + SARM1 ASO. (B3-4) 20 ctrl + ctrl ASO, 14-16 KO + ctrl ASO, 14 ctrl + SARM1 ASO, and 20-22 KO + SARM1 ASO, (ctrl + SARM1 ASO vs KO + SARM1 ASO) **p = 0.0049, (ctrl + ctrl ASO vs KO + ctrl ASO) **p = 0.0022. (C3-4) 24-25 ctrl + ctrl ASO, 16-19 KO + ctrl ASO, 20-22 ctrl + SARM1 ASO, and 22-26 KO + SARM1 ASO, **p = 0.001. One-way or two-way ANOVA with Tukey's multiple comparisons test and Kruskal-Wallis test with Dunn's multiple comparisons test were used; see details in supplemented Excel. All above data represent mean ± SEM, ****p < 0.0001, ^####p < 0.0001. Numbers of independent culture experiments are listed in Sup. Table