Figure 2.

IGF signaling: Effect of XENT and ENZA, alone or in combination, on IGF-1R and AKT phosphorylation status in VCaP cells. Cells were incubated with inhibitors in FBS-containing medium (in the absence of androgen or growth factor supplementation). Whole-cell lysates were prepared at indicated time points post-treatment and assessed by Western blot analysis. Quantitative analysis of protein bands was performed using the image analysis software ImageQuant TL 8.1.