Table 1.
Primer list
|
Name
|
Sequence
|
Company performing synthesis
|
| CagA | F: ACCCCTAGTCGGTAATG | Shanghai Invitrogen Biotech Co., Ltd. |
| R: GCTTTAGCTTCTGATACTGC | ||
| VacAs1a | F: GTCAGCATCACACCGCAAC | Shanghai Generay Biotech Co., Ltd. |
| R: CTGCTTGAATGCGCCAAAC | ||
| 16sRNA | F: CTGGAGAGACTAAGCCCTCC | Shanghai Invitrogen Biotech Co., Ltd. |
| R: AGGATCAAGGTTTAAGGATT | ||
| IL-6 | F: GCAGAAAAAGGCAAAGAATC | Wuhan Genecreate Biotech Co., Ltd. |
| R: CTACATTTGCCGAAGAGC | ||
| IL-8 | F: CACCGGAAGGAACCATCTCA | Wuhan Genecreate Biotech Co., Ltd. |
| R: TGGCAAAACTGCACCTTCACA | ||
| TNF-α | F: TCTTCTCGAACCCCGAGTGA | Wuhan Genecreate Biotech Co., Ltd. |
| R: CCTCTGATGGCACCACCAG | ||
| GAPDH | F: GGACCTGACCTGCCGTCTAG | Wuhan Genecreate Biotech Co., Ltd. |
| R: GTAGCCCAGGATGCCCTTGA | ||
| Cag1 | F: GCTATGGGGATTGTTGGGATAA | Shanghai Sangon Biotech Co., Ltd. |
| R: GCTTCAGTTGGTTCGTTGGTAA | ||
| Cag3 | F: GACACCTTGAATGTGAATGACAAA | Shanghai Sangon Biotech Co., Ltd. |
| R: GTTGTAATACCCATTGACTTGCTCTAA | ||
| CagT | F: TCTAAAAAGATTACGCTCATAGGCG | Shanghai Sangon Biotech Co., Ltd. |
| R: CTTTGGCTTGCATGTTCAAGTTGCC | ||
| CagE | F: GCGATTGTTATTGTGCTTGTAG | Shanghai Sangon Biotech Co., Ltd. |
| R: GAAGTGGTTAAAAAATCAATGCCCC | ||
| CagM | F: ACAAATACAAAAAAGAAAAAGAGGC | Shanghai Sangon Biotech Co., Ltd. |
| R: ATTTTTCAACAAGTTAGAAAAAGCC |
16sRNA: 16S ribosomal RNA; CagA: Cytotoxin-associated protein A; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; IL-6: Interleukin 6; TNF-a: Tumor necrosis factor-alpha; VacAs1a: Vacuolating cytotoxin gene As1a.