FIG. 2.

Map of ∼5-kb M. arthritidis 158p10p9 DNA insert in plasmid pM8-2 (derived from pBlueScript) containing sequences hybridizing with the oligonucleotide probe derived from the MAA2 N-terminal amino acid sequence. Base pairs are numbered beginning with the terminal EcoRI site at the 5′ end of the insert. The ORF 1 tandem direct repeat units are indicated as shaded boxes and numbered R1 to R6. A putative Rho-independent transcription terminator between ORFs 1 and 2 is indicated as a hairpin loop. Restriction sites are indicated by arrows. The ∼0.7-kb HP fragment and ∼2.2-kb, ∼0.7-kb, and ∼0.25-kb (HL, HM, and HS, respectively) fragments from this insert that were subcloned to pUC19 for DNA sequencing are also shown.