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. Author manuscript; available in PMC: 2024 Jan 29.
Published in final edited form as: J Am Chem Soc. 2021 Nov 15;143(46):19454–19465. doi: 10.1021/jacs.1c08659

Figure 3. Complete engineered biosynthesis of colchicine in N. benthamiana.

Figure 3.

Addition of the three identified enzymes to the previously engineered biosynthetic pathway to 3 allows for total biosynthesis of 1 in N. benthamiana, as shown here via LC-MS chromatograms. EIC = extracted ion chromatogram (EIC = m/z 400.1755 [M+H]+ and m/z 422.1574 [M+Na]+ for both compounds). Native Gloriosa superba genes are shown in blue, while those from other plant sources are brown.