Role of αIIbβ3 integrin activation and ligand binding in TEG. Blood samples were analyzed from WT mice (n=8) or mice with megakaryocyte/platelet-specific deletion of Talin1 (Tln1mKO, n=5). αIIbβ3 ligand binding was inhibited by treating WT samples with anti-αIIbβ3 antibody (Leo.H4, 75 μg/ml, n=6) for 10 mins prior to TEG assay. (A-C) TEG parameters: R time (A), α-angle (B) and MA (C). (D) Representative TEG traces. Data shown as mean ± SD. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparison test. Symbols directly over bars represent significance compared to control. **P< .01, ***P<0.001, ****P < .0001.