Role of PAR1/PAR4 and Syk in human blood TEG. Healthy volunteer blood samples were analyzed with addition of DMSO (n=4), vorapaxar (Vora, 5 μM, n=3), BMS-986120 (BMS, 10 μM, n=3) and PRT-2607 (PRT, 20 μM, n=4) to inhibit PAR1, PAR4 and Syk, respectively, 10 mins prior to TEG assay. (A-C) TEG parameters: R time (A), α-angle (B) and MA (C). (D) Representative TEG traces. Data shown as mean ± SD. Statistical significance was determined by one-way ANOVA with Tukey’s multiple comparison test. Symbols directly over bars represent significance compared to control. *P < .05, **P< .01, *** P<0.001, ****P < .0001.