Table 2. Site-directed mutagenesis of the Gly m Bd 30K gene using the CRISPR/Cas9 system performed with two soybean transformation platforms.

	Agrobacterium	Biolistic
Explants	Cotyledonary nodes	Somatic embryos
Soybean genotypes	Enrei, Kariyutaka	Jack
Number of explants per experiment	100∼200	Many
Selectable agents of transgenic plants	Glufosinate	Hygromycin
Period until generating T0 plants	2 months~	3 months~
Mosaicism of mutations in T1 and T2 plants	Frequent	Rare
Nucleotide characteristics in the mutant allele	Mainly deletion or insertion from one to ten-nucleotides	Deletion or insertion from one to ten-nucleotides, and insertion of more than 100-nucleotides
Frequency of obtaining Cas9-free plants in the later generations	High frequent	High frequent