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Comparison of Ras modification in HT29 cells by different P. aeruginosa strains. (A) HT29 cells were incubated with 108 CFU of strain 388, PAK, DG1, or PA103 (103), as indicated; then Ras was immunoprecipitated and detected by immunoblotting as described for Fig. 2A. M, modified Ras; U, unmodified Ras. (B) HT29 cells were incubated with 108 CFU of strain 388 or ΔT and analyzed as described above. Molecular masses (in kilodaltons) are indicated.
