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. 2023 Dec 4;43(5):363–377. doi: 10.1038/s41388-023-02897-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A heat map of a mRNA microarray analysis on ganglia cells collected from transgenic Th-MYCN ^+ /+ and wildtype mice at 1, 2, and 6 weeks of age assessing the expression of (A) all RNA splicing-related genes, or (B) the core spliceosome assembly genes. C All 26 core spliceosome assembly genes (as defined by the GO term, GO:0000387) were assessed based on the three given criteria to identify possible candidate genes. D Overall (OS) and event-free survival (EFS) multivariate CoxPH analysis using the Kocak (n = 476) neuroblastoma patient cohort was conducted on the core spliceosome assembly genes (as defined by the GO term, GO:0000387). The dot plot represents the median hazard ratios (mHR) from the CoxPH models for each gene regarding EFS and OS. E Kaplan–Meier OS curve obtained from Kocak neuroblastoma patient cohort (n = 476) dichotomised on median SNRPD3 expression. F OS multivariate CoxPH analysis on Kocak cohort (n = 649) with SNRPD3 gene expression against classic neuroblastoma prognostic factors, dots represent the median HR (hazard ratio) whilst lines represent the 95% confidence interval. G Scatter plot with a linear regression fit of mRNA expression levels from the Th-MYCN^+/+ mouse tissues for MYC-signature vs SNRPD3 gene expression (6 weeks of age, microarray, log2). H Scatter plot with linear regression fit for the Kocak neuroblastoma patient cohort for MYCN vs SNRPD3 gene expression (mRNA microarray, log2 expression). I Western blot analysis for SNRPD3, cMYC and MYCN expression in a range of MYCN-amplified (SK-N-BE(2)-C, CHP-134, KELLY, IMR-32), MYCN non-amplified (SK-N-AS, SH-SY5Y), and normal lung fibroblasts (MRC-5, WI-38) cells. GAPDH is a protein loading control. Western blot is a representative image of n = 3 independent experiments.