Figure 3.

Rhapsody pathogenicity predictions. (a) Pathogenicity probabilities calculated by Rhapsody (Rhapsody scores) demonstrate a significant correlation with experimental measurements of protein stability (calculated as in 1c). Destabilizing changes generating high Rhapsody scores are highlighted with a red circle. (b) Heatmap illustrating the results of in silico saturation mutagenesis. Each column shows the Rhapsody pathogenicity probabilities for all possible substitutions at a given residue. The series of dark green squares above the diagram represents the IL36Ra amino acid sequence, with α-helixes and β-strands highlighted by coloured bars.