Figure 5.

Validation of bioinformatic predictions for selected changes. (a) Left: representative western blot showing the accumulation of wild-type (WT) and mutant IL-36Ra, after the transfection of the relevant cDNA constructs into HeLa cells. The p.Pro27Leu disease allele was analyzed as a positive control and is highlighted in red underlined font. Right: Densitometry results. Stability was calculated as the IL-36Ra/β-actin ratio normalized to wild-type. (b) Bar plot showing the effects of selected variants on IL-36 signaling activity. All results are presented as means ± standard deviation for 3 independent transfections. ∗P<0.05; ∗∗P<0.01; ∗∗∗P<0.001.