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Expression of recombinant MSP5.0. Lanes 1 and 4 contain MSP5.0 fused to MBP and purified on an amylose affinity column; lanes 2 and 5 contain MBP alone; and lanes 3 and 6 contain a lysate of A. marginale-infected erythrocytes. Lanes 1 to 3 were reacted with rabbit antiserum against MBP; lanes 4 to 6 were reacted with the same dilution of normal rabbit serum. The positions of the MSP5.0-MBP fusion protein and MBP alone are indicated in the left margin.
