Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
MAb ANAF16C1 binding is sensitive to denaturation and reduction of MSP5. Purified MSP5.0 was either untreated or treated with 8 M urea (MSP5.0+urea); 60 mM dithiothreitol (MSP5.0+DTT); DTT and 300 mM IA (MSP5.0+DTT+IA); urea, DTT, and IA (MSP5.0+urea+DTT+IA); urea and IA (MSP5.0+urea+IA); or IA alone (MSP5.0+IA). Samples were reacted in duplicate with either MAb ANAF16C1 (left) or the negative control MAb Tryp1E1 (right).
