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. 2024 Jan 30;16:9. doi: 10.1038/s41368-023-00267-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Heterogeneity of Tregs. a Tregs were isolated from CD4 cells in single-cell data and subjected to unsupervised clustering, resulting in four distinct groups. b A violin plot depicts the expression of CXCR4 in the four Tregs clusters. Cluster 2 exhibits the highest expression of CXCR4. c A dotplot illustrates the expression of the targets panel and TGFB1 in the four Tregs clusters. Cluster 2 displays the highest expression of both. d Immunofluorescence staining shows the co-localization of CXCL12 and hypermetabolic regions (LDHA and HIF1α) in OSCC