Fig. 7.

a Biplot of PCA derived from the amino acid metabolism and developmental competence of the bovine cumulus-oocyte complexes. Vectors with close angles (< 45°) indicate a strong correlation, vectors that are perpendicular indicate no correlation, and vectors in opposite directions (approaching 180°) indicate a negative correlation. b The bivariate study employed Kendall’s tau correlation to determine the association between amino acid metabolism, heat shock, and developmental competence of COCs. Red and blue circles indicate negative and positive associations, respectively. Boxed rectangles indicate the statistical significance at P < 0.05. Blast blastocyst rate, S shock, Cleav cleavage rate