Supporting Figure S2.

A, representative images of the phagocytosis of pPSCdMs and PAMs treated with 20 μM Cytochalasin D to Green E. coli BioParticles. Positive uptake E. coli BioParticles were shown in green; cell nuclei were stained with Hoechst 33342 in blue. Scale bars represent 125 μm. B, composite bright-field and fluorescent image of Marc145 after PRRSV infection for 12  h, stained for PRRSV (green). Scale bars represent 125 μm. C, the expression level of NUP62 in pPSCdMs and PAMs after PRRSV infection for 12 h was detected by RT-qPCR. Data indicate mean ± s.d. The p values were calculated by using two-tailed t-tests. n = 3. D, the supernatant cultures of pPSCdMs and PAMs at 72 h after PRRSV infection were collected for secondary infection. Composite bright-field and fluorescent image of secondary PRRSV-infected pPSCdMs and PAMs for 12  h, stained for PRRSV (green) and PI (red). Scale bars represent 125 μm.