FIG. 2.

Immunoblot analysis of recombinant B31 Erp proteins. The filter was incubated with a 1:200 dilution of serum from a mouse infected with B31 by tick bite. The immunoblot signal strengths were greater from the recombinant ErpA/I and ErpB2/J proteins than from the other proteins, and the exposure time of ErpA/I and ErpB2/J in this figure was approximately 1/10 of that of the other Erp proteins. All of the recombinant Erp proteins migrated with apparent molecular masses that were greater than predicted from their sequences (Table 2). Some of the recombinant protein preparations contained probable degradation products or multimeric proteins, resulting in the presence of multiple bands. Molecular masses (in kilodaltons) are indicated to the left.