Fig. 2.

Regeneration following 2 mM Thymidine (cell cycle inhibitor) injection: A Control worm heals wound on 2nd day of post-amputation. B Blastema size of 0.2 mm was observed on the 4th day after post-amputation. C regenerative bud elongated to 0.3 mm on the 6th day. D blastemal size almost doubled (0.5 mm) on the 8th day upon restoration. (E) wound healing was adequately executed in Thymidine treated worm on the 2nd day of post-amputation. F blastemal growth is restricted and shows only 0.1 mm size following the 4th day of post-amputation. G unmeasurable very little blastemal growth progress was observed until the 6th day of post-amputation. H blastemal size is slightly elongated to 0.2 mm size following the 8th day of post-amputation. I histology of 8th day control blastemal tissue shows well-differentiated prostomium, mouth, septum, and elongated segments. J histology of 2 mM Thymidine injected 8th day regenerative blastema with less developed structures lacking prostomium, mouth, and with truncated regenerative body segments. Arrows (red represents septum; blue represents newly regenerated segments; yellow indicates prostomium; green represents functional mouth; purple represents intestinal tract). “D” represents the dorsal side of the earthworm, and “V” specifies the ventral surface of the earthworm