Fig. 4.

Combinatorial injection of Buclizine and Thymidine: A control worm following day-1 post-amputation at 10th segment. B the amputated worms form blastema on the 4th day. C on the 7th day, the amputated worm forms elongated blastema. D Buclizine and Thymidine injected worm following 1st day of post-amputation. E complete inhibition of regeneration on 4th day of post-amputation in Buclizine and Thymidine injected worm. F Combinatorial injected worms show no sign of regeneration even after seven days of post-amputation. G Survival graph for the Control P. excavatus, buclizine, and Thymidine double dose-treated worms. After treatment, the live and dead worms on different days are counted and plotted in the survival (Kaplan–Meier) curve. Statistical analysis was accomplished using the log-rank test (Mantel-Cox), and the obtained p values <  0.05 were considered significant. H Expression of p53, TCTP, and β-actin in 8th day Control (L1) and 8th day Buclizine and Thymidine treated samples (L2). High expression of p53 and lower expression of TCTP was observed in Buclizine and Thymidine treated samples (p53–53 kDa; TCTP − 19 kDa; Actin − 42 kDa). I Band intensity of Western blotting image is quantified and represented with a bar diagram. Statistical significance is achieved when the p value is < 0.05