FIG. 1.

Extracellular proteomes of B. subtilis 168 and Δ6. (Top) Cells of B. subtilis 168 or B. subtilis Δ6 were grown in LB medium, and extracellular proteins were harvested 1 h after entry of the cells into the post-exponential-growth phase. The extracellular proteins were separated by 2D PAGE and stained with silver nitrate. Protein spots identified by MALDI-TOF mass spectrometry are indicated. Positions on the gel of spots missing from the extracellular proteome of B. subtilis Δ6 are indicated by circles. (Bottom) B. subtilis 168 and Δ6, both containing pLip2031 for high-level production of LipA, were grown in LB medium. Secreted proteins were analyzed as described for the top panel.