TABLE 1.

Plasmids and strains

Plasmid or strain	Relevant properties	Reference
Plasmids
pMA5	pUB110 derivative; contains the HpaII promoter for the expression of cloned genes; ColE1; repB Kmr Apr	10
pLip2031	pMA5 derivative; carries the B. subtilis lipA gene under control of the HpaII promoter; Kmr	8
pKTH10	Vector containing the amyQ gene of B. amyloliquefaciens; Kmr	38
Strains
Escherichia coli
MC1061	F−araD139 Δ(ara-leu)7696 Δ(lac)X74 galU galK hsdR2 mcrA mcrB1 rspL	55
B. subtilis
168	trpC2	33
Δ1	trpC2; Δprophage1	56
Δpks	trpC2; pks::Cm; Cmr	56
TF8A	trpC2; ΔSPβ; Δskin; ΔPBSX	56
Δ5	trpC2; ΔSPβ; Δskin; ΔPBSX; Δprophage 1; pks::Cm; Cmr	56
Δ6	trpC2; ΔSPβ; Δskin; ΔPBSX; Δprophage1; pks::Cm; Δprophage 3; Cmr	56
168 lipB::pMutin2	trpC2; lipB::pMutin2; Emr	32
168 ybcC::pMutin2	trpC2; ybcC::pMutin2; Emr	32
168 ybcD::pMutin2	trpC2; ybcF::pMutin2; Emr	32
168 ybcF::pMutin2	trpC2; ybcF::pMutin2; Emr	32
168 ybcH::pMutin1	trpC2; ybcH::pMutin1; Emr	32
168 ybcI::pMutin2	trpC2; ybcI::pMutin2; Emr	32
168 ybcL::pMutin2	trpC2; ybcL::pMutin2; Emr	32
168 ybcM::pMutin2	trpC2; ybcM::pMutin2; Emr	32
168 skfA::pMutin1	trpC2; skfA::pMutin1; Emr	32
168 skfB::pMutin2	trpC2; skfB::pMutin2; Emr	32
168 skfC::pMutin2	trpC2; skfC::pMutin2; Emr	32
168 skfD::pMutin1	trpC2; skfD::pMutin1; Emr	32
168 skfE::pMutin2	trpC2; skfE::pMutin2; Emr	32
168 skfF::pMutin2	trpC2; skfF::pMutin2; Emr	32
168 skfG::pMutin2	trpC2; skfG::pMutin2; Emr	32
168 skfH::pMutin2	trpC2; skfH::pMutin2; Emr	32
TEB1030	DB430 ΔlipA ΔlipB	12
TEB1030 skfA	DB430 ΔlipA ΔlipB; skfA::pMutin1; Emr	This study
TEB1030 skfB	DB430 ΔlipA ΔlipB; skfB::pMutin2; Emr	This study
TEB1030 skfC	DB430 ΔlipA ΔlipB; skfC::pMutin2; Emr	This study
TEB1030 skfD	DB430 ΔlipA ΔlipB; skfD::pMutin1; Emr	This study
Δ1 lipB	trpC2; Δprophage1; lipB::pMutin2; Emr	This study
WB800	trpC2; nprE nprB aprE epr mpr bpf vpr wprA; Cmr Hygr	57
WB800 skfA	WB800; skfA::pMutin1; Cmr Hygr Emr	This study