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. 2024 Jan 29;15:869. doi: 10.1038/s41467-024-45238-7

Fig. 2. The paternal dosage of Cifs affects the capacity of maternal CifA to rescue incompatibility.

Fig. 2

a Construct design of β2t-cifA-cifB and topi-cifA-cifB. b Increasing the relative expression of cifA in comparison to cifB (β2t-cifA;β2t-cifA-cifB) or lowering the expression of both cif genes (topi-cifA-cifB) did not affect CI penetrance when males were crossed with wild-type (wt) females. However, it did improve the capacity of wAlbB-carrying females to rescue CI. Lines denote median and error bars interquartile ranges, numbers in parentheses denote the n. Letters indicate significant differences with an α = 0.05 calculated by a Kruskal–Wallis test: H = 209.7, P < 0.0001, d.f. = 7, followed by a two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli to correct for multiple comparisons, individual P-values are listed in the Source Data file. c The relative expression of the cifA-cifB transcript was significantly lower when the expression was regulated by the topi promoter (two-tailed unpaired t-test p < 0.0001, mean and s.d. are shown, n = 10 for both groups). Source data are provided as a Source Data file.